Molecular species analysis of phosphatidylglycerols by reversed-phase HPLC/ESI-MS
نویسندگان
چکیده
منابع مشابه
Quantitation of cyclic dinucleotides by reversed-phase LC-MS/MS
Cyclic dinucleotides such as bis-(3',5')-cyclic dimeric adenosine monophosphate (c-di-AMP) and bis-(3',5')-cyclic dimeric guanosine monophosphate (c-di-GMP) represent important second messengers in bacteria. Although their synthesis has not been described in plants so far, they may be involved in the regulation of bacterial phytopathogen-plant interactions as well as rhizobium plant symbiosis. ...
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The retention behavior of molecular species of phosphatidylcholine (PC) is studied by reversed-phase (RP) ion-pair high-performance liquid chromatography (HPLC). Mobile phases contain tetraalkyl ammonium phosphates (TAAPs) in methano-acetonitrile-water. The stationary phase is alkyl-bonded silica. Competitive interactions of TAAPs, analyte solutes, and an RP-HPLC column result in reduced retent...
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A convenient method for the separation of molecular species of complex lipids such as sphingomyelin and ceramide by argentation high performance liquid chromatography (HPLC) is described for the first time. Molecular species separation of these complex lipids was achieved for quantitative analysis and preparative work by the combined use of argentation and reversed-phase HPLC.-Smith, M., P. Mon...
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Pneumatically assisted electrospray mass spectrometry (ESMS) in the direct mode and as a chromatographic detection technique was developed for the characterization of horse kidney metallothionein isoforms. Direct analysis in an acidic medium showed the presence of three major and five minor isoforms, the molecular masses of which were determined. The presence of the major isoforms (two of which...
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In this article, we introduce a method using nanoscale ion-pair reversed-phase high-performance liquid chromatography (nano-IP-RP-HPLC) hyphenated to nanoelectrospray ionization high-resolution mass spectrometry (nano-ESI-HRMS) to separate and identify metabolites in cell extracts. Separation of metabolites was performed on a 100 μm i.d. C18 column with tributylamine (TBA) as the ion-pairing re...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 2003
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.52.795